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Lichens and eutrophication
Jadrná, Iva ; Svoboda, David (advisor) ; Malíček, Jiří (referee)
Eutrophication is a process of organic nutrients enrichment in an ecosystem, especially nitrogen and phosphorus. This bachelor thesis is dealing with the influence of eutrophication on lichens, especially with the effect of nitrogen compounds in the air and the effect of the combination of nitrogen compounds with phosphorus on epiphytic lichens. Eutrophication is defined and information is provided on changes in abundance and diversity of lichen cover. The mechanisms of eutrophication tolerance in lichens are also described. Key words: lichens, epiphytes, eutrophication, ammonia, ammonium ions, nitrogen oxides, phosphorus
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Inducible RNAi against essential genes of nitrogen metabolism as a tool for control of GM plants
Kobercová, Eliška ; Fischer, Lukáš (advisor) ; Tylová, Edita (referee)
Uncontrolled spreading of genetically modified (GM) plants is one of the main concerns about their cultivation. Inducible RNA interference against an essential gene could be a tool for control of GM plants. After spraying with a chemical inducer, the essential gene will be silenced so the treated GM plant will die. For testing this strategy we chose two key enzymes of nitrogen metabolism, glutamate synthase (GOGAT) and glutamine synthetase (GS). GS processes ammonium ions into glutamine, then GOGAT transfers the amide group from glutamine to 2-oxoglutarate to form two glutamates. GS/GOGAT cycle is the main pathway for assimilation of ammonium ions, which could be toxic to plants in a higher concentration. Disruption of ammonium assimilation during photorespiration causes a strong inhibition of photosynthesis. The aim of this work was to describe the effects of silencing GOGAT and GS genes in Arabidopsis thaliana. To induce silencing, RNAi hairpin constructs under a control of constitutive or estradiol-inducible promoter were prepared. In selected independent transformants with the inducible hairpin against GOGAT, chlorosis and reduced growth were observed after the estradiol treatment in in vitro conditions. However, the spraying with estradiol was tricky, at the whole plant level, the induction of...
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Lichens and eutrophication
Jadrná, Iva ; Svoboda, David (advisor) ; Malíček, Jiří (referee)
Eutrophication is a process of organic nutrients enrichment in an ecosystem, especially nitrogen and phosphorus. This bachelor thesis is dealing with the influence of eutrophication on lichens, especially with the effect of nitrogen compounds in the air and the effect of the combination of nitrogen compounds with phosphorus on epiphytic lichens. Eutrophication is defined and information is provided on changes in abundance and diversity of lichen cover. The mechanisms of eutrophication tolerance in lichens are also described. Key words: lichens, epiphytes, eutrophication, ammonia, ammonium ions, nitrogen oxides, phosphorus
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Determination of the quality parameters of drinking water in selected agricultural subjects in the district Jindřichův Hradec
KUČEROVÁ, Monika
The thesis deals with the issue of drinking water. The aim of the study is to evaluate quality parameters of drinking water in connection with legislation attending to this issue. The evaluation was conducted in nine agricultural subjects and analyzes of drinking water were determined and evaluated. Microbiological and hydrochemical indicators of drinking water were compared within the year 2015. Regarding microbiological indicators, coliforms, Escherichia coli, enterococci, psychrophilic bacteria were followed. Ammonia nitrogen, nitrate nitrogen, phosphate phosphor, total inorganic carbon, total organic carbon, total carbon, total nitrogen, pH, conductivity were followed regarding hydrochemical indicators. The final values showed exceeded values of the microbiological indicators at five sampling sites according to applicable legislation. Furthermore, threshold values of total organic carbon were exceeded in all tested agricultural subjects and also values of nitrate nitrogen in drinking water were increased at three subjects evaluated throughout the year.
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